A frame time of the main theoretical milestones in both theory and experimental mass spectrometry is highlighted here. A paragraph emphasises the ionisation energy issues, the basics of ionisation voltage, the developing potential and the energy balance. Isotopic percentage and nominal mass calculation are also explained along with fragmentation mechanism. First, the fundamental notions of mass spectrometry are explained, so that the reader can easily cover this chapter (graphs, main pick, molecular ion, illogical pick, nitrogen rule, etc.). The chapter includes an introduction to the main ionisation techniques in mass spectrometry and the way the resulting fragments can be analysed.
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